Table 1 Primer pairs for qPCR analysis. This table only shows the highest efficiency primer pairs, as well as the primers used to produce the purified PCR products for the standard curves
From: Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response
Transcript | Primer ID | Sequence (5′ to 3′) | Length (bp) |
|---|---|---|---|
tektin-2 | T2F_1 | CCACACCCTTCAGCAGTGT | 19 |
T2R_1 | GCGATCTTTGCGCGGATTT | 19 | |
T2F_2 | AGTTCGCCAGGAGAGTCGA | 19 | |
T2R_4 | CTCCTCTAGAGCCCTCTTCGT | 21 | |
tektin-4-like | T4F_1 | ACAATGGGGTTCAGGGCTG | 19 |
T4R_2 | CGGACGCTGACACACTTGT | 19 | |
T4F_3 | TGAGAGAATTCGCCACGAG | 19 | |
T4R_3 | TGTTGCACGGAGTCCATTT | 19 |